Kartiningsih^1,2, Deni Rahmat², Partomuan Simanjuntak³ and Syamsudin Abdillah<sup>4*

1</sup>Faculty of Pharmacy, Universitas Pancasila, Indonesia.
²Department of Pharmaceutical of Technology, Faculty of Pharmacy, Universitas Pancasila, Jagakarsa, South Jakarta 12640, Indonesia.
³Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong, Bogor, Indonesia.
⁴Department of Pharmacology, Faculty of Pharmacy, Universitas Pancasila, Indonesia.

 

The platelet anti-aggregation activity of hidrolized and irradiatiated crude fucoidan from brown sea weed (Sargassum polycystum) was investigated in this study. The crude fucoidan of the brown seaweed was obtained by maceration using 2% CaCl2 (w/v). The platelet anti-agregation activity test was carried out using the modified LTA method. This study aimed at examining platelet anti-aggregation activity of hydrolyzed and irradiated fucoidan. Thereafter, it was degraded by gamma irradiation in doses of 20, 40 and 60 kGy, respectively. Furthermore, hydrolysis with HCl (4, 6 and 8%) and cellulose enzyme (20, 40 and 60 µl) were also used to degrade fucoidan. The platelet anti-aggregation activity test was carried out using the modified LTA method. The results showed that the lowest molecular weight of degraded fucoidan obtained by gamma irradiation of 20 kGy was 753.16 Da and resulted in activity percentage of platelet anti-aggregation of 75.44%. Molecular weight of fucoidan hydrolyzed by HCl 8% was 714.48 Da, while its platelet anti-agregation activity was 61.91%. In addition, the cellulase enzyme hydrolysis of 60 µl produced degraded fucoidan with molecular weight of 714.67 and its platelet anti-agregation activity was 64.30%. It was concluded that the molecular weight reduction of fucoidan molecules by gamma irradiation demonstrated the highest platelet anti-aggregation activity.

Key words: Brown seaweed, crude fucoidan, HCl hydrolysis, cellulase enzyme, gamma irradiation, platelet anti-agregation.