Sufentanil protects rat myocardium against ischemia-reperfusion injury through
miR-1 and Bcl-2 pathway
Accepted 24th October, 2018
Qiao Qiao1, Tiao li2,
Yaoxing Gao1* and Pengwei Zhao3*
1Affiliated Hospital of Inner
Mongolia Medical University, China. 2Tarim Univerisity, China. 3Inner Mongolia Medical University,
China.
Sufentanil, a lipophilic opioid, has been
clinically used to treat patients with ischemic
heart disease. The effects of sufentanil on the
miR-1 and Bcl-2 pathways were explored in the
context of ischemic heart disease. The effects
of sufentanil were assessed in rats using an
ischemic reperfusion (IR)-induced myocardium
injury rat model. Sixty rats (body weight of 200
g ± 30 g) of either sex were randomly assigned
to three groups (a sham operation group (SO
group), an ischemia/reperfusion group (IR
group), and an IR group treated with sufentanil
(SUF group). Experiments were conducted to
assess CK, LDH, MDA, SOD, Ca2+-Mg2+-ATPase, and
Na+-K+-ATPase. qPCR was used to detect miR-1
expression. Protein levels of Bcl-2, Bax,
Caspase 3, Caspase 8 and Caspase 9 were measured
by western blot analysis and the protein
expression of Bcl-2, Bax, Caspase 3, Caspase 8
and Caspase 9 were measured. The effect of
sufentanil on the growth of H9C2 myocardium
cells was determined in vitro. Serum CK, LDH and
MDA increased in the IR group, while the SOD,
Ca2+-Mg2+-ATPase, and Na+-K+-ATPase levels of
the IR group reduced. Expression levels of
miR-1, Bax, Caspase 3, Caspase 8 and Caspase 9
increased in the IR group, but the expression
level of Bcl-2 did not increase. In H2C9 cells,
the abundance of Bcl-2 was increased at 0, 4 and
8 h, but the abundance of miR-1, Bax, Caspase 3,
Caspase 8, and Caspase 9 between 8 and 12 h time
points decreased. Over-expression of miR-1 did
not alter expression levels of Bax, Caspase 3,
Caspase 8, or Caspase 9. Sufentanil (10 μM)
accelerated H9C2 cell growth. When Bcl-2 was
silenced, miR-1 expression was reduced in H9C2
cells after 0, 4 and 8 h of growth; however,
expression of Bax, Caspase 3, Caspase 8 and
Caspase 9 did not change. The growth rate of
H9C2 cells exposed to sufentanil was
significantly more rapid than that of untreated
H9C2 cells. The therapeutic effects of
sufentanil in patients with ischemic heart
disease are at least partially mediated by
changes in signaling through the miR-1 and Bcl-2
pathways.
Key words:
Sufentanil, Ischemia, miR-1, Bcl-2.
This is an open access article
published under the terms of the
Creative Commons Attribution
License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is
properly cited.
Cite this article as: Qiao Q, li T, Gao Y, Zhao P (2018). Sufentanil
protects rat myocardium against ischemia-reperfusion injury through miR-1 and
Bcl-2 pathway. J. Biol. Ser. 1(4): 148-155.
