Towards
development of new technique for cryopreservation of date palm (Phoenix
dactylifera L.) in vitro grown cultures
Accepted 14th November, 2018
Abdullatif A. Al-Khateeb1, Mohei
EL-Din Solliman1,2, Heba Allah A.
Mohasseb1,2, Suliman A. Al-Khateeb3,
Mohammed I. Aldaej1 and Wael F.
Shehata1,4
1Plant Biotechnology Department,
College of Agricultural and Food Sciences, King
Faisal University, P.O. Box 420, Alhassa 31982,
Kingdom of Saudi Arabia. 2Plant Biotechnology Department,
National Research Centre, Dokki – Egypt, 12622,
Cairo. 3Environment and Natural Resources
Department, College of Agriculture and Food
Sciences, King Faisal University, P.O. Box 400,
Alhassa 31982, Kingdom of Saudi Arabia. 4Plant Production Department, College
of Environmental Agricultural Science, El-Arish
University, North Sinai, Egypt.
The method of cryopreservation was described as
very simple with the potential application of a
wide range of date palms. The undifferentiated
tissue cultures have been successfully frozen by
later freezing methods of renewed seedlings.
Shoot tips of date palm were cultured on
MS-medium containing 10 mg / l 2,4-D + 3mg / l
2iP. The potential of dehydration caused by air
drying to cryopreservation of date palm tissue
cultures through direct immersion in liquid
nitrogen was subjected. The embryogenic callus
of about 65% of the water content caused by 20
min of air drying period recorded the highest
percentage of survival and in vitro conversion
to plants. Among the different types of sugars
(sucrose) used as pre-treatment agents in the
pre-plant average, sucrose was the best for the
persistence of cryogenic palm tree cultures. The
highest survival rate (80%) and switch to plants
(75%) were observed with 1 M sucrose.
Cryopreservation of date palm embryogenic callus
through encapsulation is a new method we tried
to introduce to our laboratory and spread for
others also. Date palm (Phoenix
dactylifera
L) tree is a member of the family Palmace (Arecaceae)
and inhabit tropical and sub-tropical habitat.
The Arecaceae is a family of woody perennial
monocots and consists of about 200 genera and
2500 species. The entire tree of date palm is
utilized to provide food, fiber, fuel and
furniture. Date palm trees are tolerance to
environmental stresses ranging from salinity to
drought and high temperature, in addition to low
maintenance and yield harvesting costs. The
encapsulation is a simple process that can be
done inserting the propagule singly into an
alginate drop and then falling it into a
complexing solution (20 to 35 min) to form
calcium alginate capsules that are approximately
5 mm in diameter. The presence of nutritive
elements into the capsule (artificial endosperm)
has a function similar to endosperm of zygotic
seeds. RAPD was extensively used to study the
genetic stability of cryogenic tissue cultures
from date palm. According to RAPD analysis, the
seedlings derived from cryopreserved cultures
were similar to those derived from untreated
cultures, both of which were similar to the
cultivated plants in the field. Finally, whole
plants of chilled cultures were able to adapt to
free living conditions after a stage of
adaptation.
Key words:
Embryogenesis, Khalas, Date palm,
cryopreservation, vitrification, RAPD analysis.
This is an open access article
published under the terms of the
Creative Commons Attribution
License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is
properly cited.
Cite this article as:
Al-Khateeb AA, Solliman MED, Mohasseb HAA, Al-Khateeb
SA, Aldaej MI, Shehata WF (2018). Towards development of new technique for
cryopreservation of date palm (Phoenix
dactylifera
L.)
in vitro
grown cultures. J. Biol. Ser. 1(4): 138-147.
