1Development Research Depratment,
Snowden Co., Ltd., Tokyo 101-0032, Japan. 2Department of Psychiatry, Tokai
University School of Medicine, Kanagawa
259-1193, Japan. 3Center for Sustainable Resource
Science, RIKEN, Saitama 351-0198, Japan.
Novel bacilli producing anti-Helicobacter
pylori
compounds were screened from natural
sources, and a new strain,
Bacillus subtilis
A81, was found to have strong anti-Helicobacter
pylori
activity. To determine the molecular structure
of the anti-Helicobacter
pylori
compound(s) in
Bacillus subtilis
A81 cultures, we fractionated the culture
supernatant containing activities with solvent
extraction and silica gel chromatography. By
analyzing with 1H and 13C nuclear magnetic
resonance analyses, one compound was identified
to be methyl 4-hydroxybenzoate (methylparaben).
Helicobacter pylori
was found to be relatively more susceptible
(10-fold or more) to methylparaben than other
bacteria, Staphylococcus
aureus
and
Escherichia coli.
To our knowledge, this might be the first report
describing methylparaben as a natural anti-Helicobacter
pylori
product derived from
B. subtilis.
Key words:
anti-Helicobacter
pylori
activity,
Bacillus subtilis,
methyl 4-hyroxybenzoate, methylparaben, nuclear
magnetic resonance, silica gel column
chromatography.
This is an open access article
published under the terms of the
Creative Commons Attribution
License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is
properly cited.
Cite this article as:
Aiba Y, Hiratsuka T, Nishizuka T, Koshino H,
Yoshida M, Komatsu Y (2018). Identification of methyl 4-hydroxybenzoate as a
natural anti-Helicobacter
pylori
agent from the culture broth of
Bacillus subtilis
A81. Acad. J. Sci. Res. 6(4): 134-139
