Topical Application
of Naringenin inhibits Hypertrophic Scar formation by Suppressing Fibroblast
Activation and Local Inflammation.
Accepted 11th
March, 2016
Shengzhou Shana,1, Yifan Zhanga,1,
Min Wua, Bo Yib, Jing Wanga,1* and Qing Feng Lia*.
aDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth
People’s Hospital, Shanghai Jiao Tong University School of Medicine, 639
Zhizaoju Road, 200011 Shanghai, China.
bClinical College of General Hospital of Beijing Military Region,
Anhui Medical University, Hefei, China.
The pathogenesis and
therapy of hypertrophic scar (HS) have not yet been established. The aim of our
study is to investigate the potential effect of naringenin on HS and its
underlying mechanisms. The mouse model with HS was prepared by mechanical
stretch device and then treated with naringenin at various concentrations.
Histological studies were performed to evaluate scar hypertrophy by haematoxylin
and eosin and Masson's trichrome staining. The activation of HS fibroblasts was
determined based on real-time PCR, Western blot and immune-histochemical
staining. After observing the retention of inflammation cells by immune-histochemistry,
the cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1beta
(IL-1β), interleukin-6 (IL-6) and transforming growth factor-beta 1 (TGF-β1),
mRNA and protein levels were quantitated by real-time PCR, ELISA and Western
blot methods. Naringenin significantly inhibited the formation of HS in a
concentration-dependent manner. Naringenin also inhibited fibroblasts activation
and inflammatory cells recruitment. In addition, mRNA and protein expression
levels of TNF-α, IL-1β, IL-6 and TGF-β1 were down-regulated after naringenin
treatment. This study highlighted new pharmacological activity of naringenin on
HS. The mechanism of action of naringenin was associated with the inhibition of
fibroblast activation and local inflammation. These results suggested that
naringenin could serve as a novel agent for treatment of HS.
This is an open
access article published under the terms of the
Creative
Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the
original work is properly cited.
Cite this article as:
Shan S, Zhang Y, Wu M, Yi B, Wang J, Li QF (2016). Topical Application of
Naringenin inhibits Hypertrophic Scar formation by Suppressing Fibroblast
Activation and Local Inflammation. Acad. J. Pharm. Pharmacol. 4(1): 016-023.
