The effect of essential oil of Hyptis crenata on
sepsis-induced hypothalamus injury in rats
Accepted 10th
June, 2020
Lima GC1,2, Araújo PCC2, Silva RO3,
Soares PMG4, Portella VG2, de
Souza ANC2 and Diniz LRL5*
1Laboratory of Marine Algae Proteins and
Carbohydrates, Department of Biochemistry and Molecular
Biology, Federal University of Ceará, Fortaleza, Ceará,
Brazil. 2Medical School, State University of Ceará,
Fortaleza, Ceará, Brazil. 3Department of Biomedicine, University Center
INTA – UNINTA, Sobral, Ceará, Brazil. 4Laboratory of Physiopharmacology Studies on
Gastrointestinal Tract, Department of Morphology,
Federal University of Ceará, Fortaleza, Ceará, Brazil. 5Department of Physiology, Federal University
of Sergipe - CEP 49100-000, São Cristóvão, Sergipe,
Brazil.
Hypothalamic impairments are associated with severe
clinical complications caused by sepsis, including
insulin resistance, heart rate variability losses and
autonomic dysfunction. Studies have shown that
neuroinflammation and oxidative stress are critical to
the pathogenesis of neurological disorders. Essential
oils have been used to treat neurological disturbances.
Previous studies have shown that the essential oil of
Hyptis crenata (EOHc), which belongs to the lamiaceae
family, has significant anti-inflammatory and
antioxidant properties. The aim of present study was to
evaluate the effect of EOHc on hypothalamic injury
induced by sepsis. Wistar rats were subjected to Sham
and CLP surgical procedures. EOHc (300 mg/kg) was orally
administered 12 and 24 h after sepsis induction. The
animals were euthanized 48 h after surgery and their
hypothalamus were removed. TNF-α, IL-β and IL-6 levels
in the hypothalamus were measured through ELISA.
Malondialdehyde levels and catalase activity were
measured to assess oxidative stress. The morphology of
the hypothalamus was analyzed by means of HE staining.
EOHc inhibited TNF-α and IL-β level increase in the
hypothalamus, inflammatory cell infiltration, epithelial
cell loss and lipid peroxidation in CLP – operated rats
as compared with the control group. Data in the current
study evidenced EOHc effectiveness to help rule out
hypothalamus sepsis-induced injury.
This is an open access article
published under the terms of the
Creative Commons Attribution
License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is
properly cited.
Cite this article as: Lima GC, Araújo PCC,
Silva RO, Soares PMG, Portella VG, de Souza ANC, Diniz LRL (2020). The effect of
essential oil of Hyptis crenata on sepsis-induced hypothalamus injury in
rats. Acad. J. Med. Plants. 8(7): 088-095.
