Academia Journal of Agricultural Research 3(12):
381-394, December 2015
DOI: 10.15413/ajar.2015.0177
ISSN: 2315-7739
2015 Academia Publishing
Abstract
Genetic diversity analysis of a local Date palm
pollinators collection (Phoenix
dactylifera L.)
using SSR Markers and study of their metaxenic
effects on the maturation and quality of Dates
obtained.
Accepted 3rd November, 2015
*Kadri Karim1,4, Barrani Halima1, Chatti Khaled2,
Kadri Nabila3 and Ben Hmida Foued3
1Centre regional de recherch en agriculture oasienne de Degache,
Laboratoire des ressources gntiques et biotechnologie vgtale, route de
Tozeur km1, BP 62, 2260 Degache Tunisia. 2Institut Suprieur de Biotechnologie de Monastir, Avenue de Taher
Hadded - B.P. n74 -Monastir -5000, Tunisia. 3Centre Technique des Dattes, route Gabes, BP 20,4280 - Kbili –
Tunisie 4National Institute of Agronomic Research of Tunis. Laboratory of
Biotechnology applied to agriculture. (Tunisia), 2049, Ariana, Tunisia.
The effect of pollen on
the maturity and the quality of the fruits of the variety "Deglet Nour" was
evaluated in this study. The results showed that the duration of maturation and
the quality of fruit obtained vary depending on pollinator. P10 is the most
efficient and this thanks to its ameliorative effect on fruit quality and
advancement of maturation. The “Bisser” stage was reached 180 days after
pollination and lasted from 3 to 4 weeks depending on the pollinator. The “Tamar
“stage started after 130 days greater or lesser seven days. The stage lasted 4
to 6 weeks. Selection for maturation earliness was already possible at the
“Bisser” stage. Molecular typing of these 10 pollinators was performed using
microsatellite or SSR analysis. A total of 22 alleles corresponding to 6
targeted microsatellite loci were generated, which provides evidence for a
significant DNA diversity among Tunisian date- palms. Using those microsatellite
markers not only highlighted the intra cultivar homogeneity, but allowed for
genetic fingerprinting of the ecotypes. The data suggests that there is a
specific consensus SSR profile for all pollinators. Moreover, by using multiloci
genotyping with only 3 microsatellites, a varietal identification key was
created allowing for perfect discrimination. These 6 new co-dominant markers
will be a starting point for researchers making use of the markers for genetic
mapping and diversity analysis of date palm.
Key words:
Phoenix dactylifera L.,
pollinator, metaxenic effect, SSR markers, MDSCALE.
This is an open access article
published under the terms of the
Creative Commons Attribution
License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is
properly cited.
Cite this article as:
Karim K, Halima B, Khaled C, Nabila K, Foued BH
(2015). Genetic diversity analysis of a local
Date palm pollinators collection (Phoenix
dactylifera L.) using SSR Markers and study of
their metaxenic effects on the maturation and
quality of Dates obtained. Acad. J. Agric. Res.
3(12): 381-394
