1Department of Health Food, Chung Chou University of Science and
Technology, Changhua, Taiwan. 2Department of Boindustry Technology, Da-Yeh University, Changhua,
Taiwan.
*Corresponding author e-mail: ccwlly@gmail.com
The protein isolates
from bee pupae were hydrolyzed by Alcalase and Flavourzyme, and the angiotensin
I converting enzyme (ACE) inhibitory activities of the hydrolysates were
evaluated. The results indicated that hydrolysis could increase the ACE
inhibitory activity; the maximum value of the inhibition was 66.71% (IC50 =
22.70 mg/ml). The hydrolysates with high ACE inhibitory activity were also
fractionated by different molecular weight cut-off (MWCO) membrane ultra
filtration. The ACE inhibitory activities of the filtrates of MWCO 5~3 kDa, 3~1
kDa and < 1 kDa were found to be 69.77, 61.53 and 96.98%, respectively, and the
IC50 were 20.04, 31.78 and 0.66 mg/ml, respectively. The results suggest that
the bee pupae hydrolysates can be used as an ingredient for nutraceuticals
against hypertension and its related diseases.
This is an open access article
published under the terms of the
Creative Commons Attribution
License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is
properly cited.
Cite this article as:
Chen CW, Chai YU, Tu CC,
Cheng MC, Tseng CY, Chang CY (2015). Angiotensin
I converting enzyme inhibitory activity of bee
pupae protein hydrolysates. Acad. J. Agric. Res.
3(12): 361-368.
